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通过柱色谱法分离迟发型超敏反应中具有活性的细胞。

Separation by column chromatography of cells active in delayed-onset hypersensitivities.

作者信息

Godfrey H P, Gell P G

出版信息

Immunology. 1976 May;30(5):695-703.

PMID:776818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1445043/
Abstract

Lymph node cells from guinea-pigs contact sensitive to 1-thiocyamo-2,4-dinitrobenzene have been fractionated by affinity chromatography over modified polyacrylamide beads. Cells mediating lymphokine release in response to active sensitizer were depleted only by chromatography over dinitrophyenyl (DNP) containing substrates and could be specifically eluted with DNP-glycine. DNP rosette-forming cells (RFC) were equally well depleted by chromatography using either DNP or trinitrophenyl containing materials but could not be eluted from the columns by DNP-glycine. While the antigen receptors of cells mediating the release of macrophage agglutination factor in response to DNP-containing antigens and of DNP-RFC were found to be hapten-specific, their specificity was shown to differ using chromatography over trinitrophenyl containing polyacrylamide.

摘要

对1-硫氰基-2,4-二硝基苯接触敏感的豚鼠淋巴结细胞，已通过在修饰的聚丙烯酰胺珠上进行亲和层析进行了分离。响应活性致敏剂介导淋巴因子释放的细胞仅通过在含二硝基苯（DNP）的底物上进行层析而被耗尽，并且可以用DNP-甘氨酸特异性洗脱。使用含DNP或三硝基苯基的材料进行层析时，DNP玫瑰花结形成细胞（RFC）同样能很好地被耗尽，但不能用DNP-甘氨酸从柱上洗脱下来。虽然发现响应含DNP抗原介导巨噬细胞凝集因子释放的细胞和DNP-RFC的抗原受体是半抗原特异性的，但使用含三硝基苯基的聚丙烯酰胺进行层析时，显示它们的特异性有所不同。

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引用本文的文献

1
Ability of an anti-T-cell serum to dissociate two features of cellular hypersensitivity in the guinea-pig.一种抗T细胞血清解离豚鼠细胞超敏反应两个特征的能力。
Immunology. 1980 Jun;40(2):247-53.
2
The use of 1-fluoro-2,4-dinitrobenzene as an affinity label for the antigen receptor of delayed hypersensitivity.使用1-氟-2,4-二硝基苯作为迟发型超敏反应抗原受体的亲和标记物。
Immunology. 1976 Oct;31(4):665-73.

本文引用的文献

1
STUDIES ON THE SPECIFICITY OF THE CELLULAR INFILTRATE IN DELAYED HYPERSENSITIVITY REACTIONS.迟发型超敏反应中细胞浸润特异性的研究
J Immunol. 1963 Mar;90:466-77.
2
Studies on hypersensitivity. IV. The relationship between contact and delayed sensitivity: a study of the specificity of cellular immune reactions.超敏反应研究。IV. 接触性超敏反应与迟发型超敏反应之间的关系：细胞免疫反应特异性的研究
J Exp Med. 1961 Mar 1;113(3):571-85. doi: 10.1084/jem.113.3.571.
3
The effect of physical and chemical properties of the sensitizing substance on the induction and elicitation of delayed contact sensitivity.致敏物质的物理和化学性质对迟发型接触性过敏反应诱导和激发的影响。
J Immunol. 1971 Feb;106(2):431-41.
4
Receptors on immunocompetent cells. I. Receptor specificity of cells participating in a cellular immune response.免疫活性细胞上的受体。I. 参与细胞免疫应答的细胞的受体特异性。
Cell Immunol. 1970 Oct;1(4):404-18. doi: 10.1016/0008-8749(70)90017-1.
5
In vitro studies of cell-mediated immunity. I. Induction of lymphocyte transformation by a soluble "mitogenic" factor derived from interaction of sensitized guinea-pig lymphoid cells with specific antigen.细胞介导免疫的体外研究。I. 致敏豚鼠淋巴细胞与特异性抗原相互作用产生的可溶性“促有丝分裂”因子诱导淋巴细胞转化
Immunology. 1970 Apr;18(4):599-610.
6
The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents.交联聚丙烯酰胺微球的衍生化。为制备特殊用途的生化吸附剂而对官能团进行的可控引入。
Biochemistry. 1969 Oct;8(10):4074-82. doi: 10.1021/bi00838a026.
7
Receptors on immunocompetent cells. 3. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 125 I-binding cells of immunized guinea pigs.免疫活性细胞上的受体。3. 免疫豚鼠的二硝基苯基化豚鼠白蛋白-¹²⁵I结合细胞上受体的特异性和性质。
J Exp Med. 1971 Aug 1;134(2):517-31. doi: 10.1084/jem.134.2.517.
8
Cutaneous basophil hypersensitivity. 3. Participation of the basophil in hypersensitivity to antigen-antibody complexes, delayed hypersensitivity and contact allergy. Passive transfer.皮肤嗜碱性粒细胞超敏反应。3. 嗜碱性粒细胞在对抗抗原-抗体复合物的超敏反应、迟发型超敏反应及接触性过敏中的作用。被动转移。
J Immunol. 1971 Jul;107(1):138-48.
9
Separation of normal and immune lymphoid cells by antigen-coated coated columns. Antigen-binding characteristics of membrane antibodies as analyzed by hapten-protein antigens.通过抗原包被柱分离正常和免疫淋巴细胞。通过半抗原-蛋白质抗原分析膜抗体的抗原结合特性。
J Exp Med. 1970 Jul 1;132(1):110-26. doi: 10.1084/jem.132.1.110.
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Specific separation of cells on affinity columns.亲和柱上细胞的特异性分离。
Proc Natl Acad Sci U S A. 1970 Jul;66(3):685-92. doi: 10.1073/pnas.66.3.685.

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