Cyong J, Okada H
Immunology. 1976 May;30(5):763-7.
Generation of fatty acids by cell-mediated immune reactions was demonstrated histochemically by Okamoto's method, which stains fatty acids and their alkaline earth salts red-brown. The red-brown deposits were observed more frequently on the mixed culture of immune lymph node cells of C3H/He and C57Bl/6 mice than on the culture of either of the pair alone. Similar evidence was observed in the reaction of immune lymph node cells with the corresponding allogeneic mouse tumour cells as well as on the immune lumphocytes of guinea-pig incubated in the presence of the soluble antigen PPD. In spite of the findings which indicated a possible generation of fatty acids in the immunological reaction of immune lymphocytes, we failed to demonstrate evidence of fatty acid generation in the ascites during rejection of allogeneic ascitic MM102 tumour cells, although accumulation of neutral lipid was evident. This unexpected contradicition was interpreted by finding that MM102 cells incubated with oleic acid did not contain fatty acid but did contain a remarkable amount of neutral lipid on histochemical examinations. This finding indicated that once generated, fatty acids would be converted to neutral lipid in the ascites where allogeneic tumour cells were rejected.
冈本氏法通过组织化学方法证实了细胞介导的免疫反应可生成脂肪酸，该方法能将脂肪酸及其碱土金属盐染成红棕色。在C3H/He和C57Bl/6小鼠免疫淋巴结细胞的混合培养物中，比单独培养这两种细胞中的任何一种时，更频繁地观察到红棕色沉积物。在免疫淋巴结细胞与相应的同种异体小鼠肿瘤细胞的反应中，以及在可溶性抗原PPD存在下孵育的豚鼠免疫淋巴细胞中，也观察到了类似的证据。尽管这些发现表明免疫淋巴细胞的免疫反应中可能会生成脂肪酸，但我们未能在同种异体腹水MM102肿瘤细胞排斥反应期间的腹水中证明脂肪酸生成的证据，尽管中性脂质的积累很明显。通过组织化学检查发现，与油酸孵育的MM102细胞不含脂肪酸，但含有大量中性脂质，这一意外的矛盾得到了解释。这一发现表明，一旦生成，脂肪酸会在排斥同种异体肿瘤细胞的腹水中转化为中性脂质。
