Oleszak E L, Kuzmak J, Hogue B, Parr R, Collisson E W, Rodkey L S, Leibowitz J L
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Hybridoma. 1995 Feb;14(1):1-8. doi: 10.1089/hyb.1995.14.1.
We have previously demonstrated molecular mimicry between the S peplomer protein of mouse hepatitis virus (MHV) and Fc gamma R (Fc gamma R). A monoclonal antibody (MAb) to mouse Fc gamma R (2.4G2 anti-Fc gamma R MAb), purified rabbit immunoglobulin, but not their F(ab')2 fragments, as well as mouse and rat IgG, immunoprecipitated (1) recombinant S peplomer protein expressed by a vaccinia virus recombinant in human, rabbit, and mouse cells, and (2) natural S peplomer protein from cells infected with several strains of MHV and MHV escaped mutants. We report here results of studies documenting molecular mimicry between Fc gamma R and S peplomer protein of viruses representing three distinct antigenic subgroups of the Coronaviridae. We have shown a molecular mimicry between the S peplomer protein of bovine corona virus (BCV) and Fc gamma R. The 2.4G2 anti-Fc gamma R MAb, rabbit IgG, but not its F(ab')2 fragments, as well as homologous bovine serum, free of anti-BCV antibodies, immunoprecipitated S peplomer protein of BCV (Mebus strain). In contrast, we did not find molecular mimicry between S peplomer protein of human corona virus (HCV-OC43) and Fc gamma R. Although the OC43 virus belongs to the same antigenic group as MHV and BCV, MAb specific for human Fc gamma RI or Fc gamma RII and purified human IgG1, IgG2, and IgG3 myeloma proteins did not immunoprecipitate the S peplomer protein from HCV-OC43-infected RD cells. In addition, we did demonstrate molecular mimicry between the S peplomer protein of porcine transmissible gastroenteritis virus (TGEV) and Fc gamma R. TGEV belongs to the second antigenic subgroup of coronaviridae.(ABSTRACT TRUNCATED AT 250 WORDS)
我们之前已经证明了小鼠肝炎病毒（MHV）的S刺突蛋白与FcγR之间存在分子模拟。一种针对小鼠FcγR的单克隆抗体（2.4G2抗FcγR单克隆抗体）、纯化的兔免疫球蛋白，但不是它们的F(ab')2片段，以及小鼠和大鼠IgG，能够免疫沉淀：（1）由痘苗病毒重组体在人、兔和小鼠细胞中表达的重组S刺突蛋白，以及（2）来自感染了几种MHV菌株和MHV逃逸突变体的细胞的天然S刺突蛋白。我们在此报告了一些研究结果，这些研究记录了代表冠状病毒科三个不同抗原亚组的病毒的FcγR与S刺突蛋白之间的分子模拟。我们已经显示了牛冠状病毒（BCV）的S刺突蛋白与FcγR之间存在分子模拟。2.4G2抗FcγR单克隆抗体、兔IgG，但不是其F(ab')2片段，以及不含抗BCV抗体的同源牛血清，能够免疫沉淀BCV（Mebus株）的S刺突蛋白。相比之下，我们未发现人冠状病毒（HCV-OC43）的S刺突蛋白与FcγR之间存在分子模拟。尽管OC43病毒与MHV和BCV属于同一抗原组，但针对人FcγRI或FcγRII的单克隆抗体以及纯化的人IgG1、IgG2和IgG3骨髓瘤蛋白不能免疫沉淀来自HCV-OC43感染的RD细胞的S刺突蛋白。此外，我们确实证明了猪传染性胃肠炎病毒（TGEV）的S刺突蛋白与FcγR之间存在分子模拟。TGEV属于冠状病毒科的第二个抗原亚组。（摘要截短于250字）
