土曲霉中衣康酸的生物合成
Itaconate biosynthesis in Aspergillus terreus.
作者信息
Bonnarme P, Gillet B, Sepulchre A M, Role C, Beloeil J C, Ducrocq C
机构信息
Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Gif sur Yvette, France.
出版信息
J Bacteriol. 1995 Jun;177(12):3573-8. doi: 10.1128/jb.177.12.3573-3578.1995.
Abstract
Itaconate biosynthesis was studied in intact cells of high-yield (RC4') and low-yield (CM85J) strains of the fungus Aspergillus terreus by methods (tracers, nuclear magnetic resonance spectroscopy, and mass spectroscopy) that did not interfere with metabolism. Itaconate formation in RC4' required de novo protein biosynthesis. Krebs cycle intermediates increased in both strains during the production of itaconic acid. The Embden-Meyerhof-Parnas pathway and the Krebs cycle were shown to be involved in this biosynthesis by using 14C- and 13C-labelled substrates and nuclear magnetic resonance spectroscopy. A metabolic pathway for itaconate formation from glucose in A. terreus is proposed.
摘要
通过不干扰代谢的方法（示踪剂、核磁共振光谱和质谱），对高产（RC4'）和低产（CM85J）土曲霉菌株的完整细胞中的衣康酸生物合成进行了研究。RC4'中衣康酸的形成需要从头进行蛋白质生物合成。在衣康酸生产过程中，两种菌株中的三羧酸循环中间产物均增加。通过使用14C和13C标记的底物以及核磁共振光谱，表明糖酵解途径和三羧酸循环参与了这种生物合成。提出了土曲霉中由葡萄糖形成衣康酸的代谢途径。
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