Payne D, Hoskins S, Schouten H, van Vleuten H, Tyring S
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1029, USA.
J Virol Methods. 1995 Mar;52(1-2):105-10. doi: 10.1016/0166-0934(94)00148-a.
Polymerase chain reaction (PCR) buffers were optimized for the specific detection of human papillomavirus (HPV) sequences. The effect of pH, potassium chloride concentration and magnesium chloride concentration of three different consensus primers were examined. Several phylogenetically distinct HPVs (HPV1, HPV2, HPV6, HPV8, HPV16, HPV18, and HPV20) were used to determine the optimal buffer components. Genital types were less sensitive to changes in pH than cutaneous types. Higher buffer pH, with a few exceptions, led to increased sensitivity and specificity of HPV detection.
聚合酶链反应（PCR）缓冲液针对人乳头瘤病毒（HPV）序列的特异性检测进行了优化。研究了三种不同共有引物的pH值、氯化钾浓度和氯化镁浓度的影响。使用了几种系统发育上不同的HPV（HPV1、HPV2、HPV6、HPV8、HPV16、HPV18和HPV20）来确定最佳缓冲液成分。生殖器型HPV对pH值变化的敏感性低于皮肤型。除了少数例外情况，较高的缓冲液pH值会提高HPV检测的灵敏度和特异性。
