Uhing M R, Kimura R E
Department of Pediatrics, Rush Medical College, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA.
J Clin Invest. 1995 Jun;95(6):2790-8. doi: 10.1172/JCI117983.
The effects of surgical bowel manipulation and anesthesia on intestinal glucose absorption were determined in chronically catheterized rats. Total and passive rates of glucose absorption were measured using 3-O-methyl-glucose (3OMG) and L-glucose, metabolically inert analogues of D-glucose. The rates of 3OMG absorption immediately postoperative and 4 h later were 86 and 62% less than the absorption rate 6 d postoperative. The absorption rates of 3OMG 1 and 2 d postoperative were not different from 6 d postoperative. Absorption of L-glucose was not altered by bowel manipulation and anesthesia. Even after correction for the increased resistance of the unstirred water layer (UWL) after bowel manipulation, the rates of total and active intestinal glucose absorption immediately postoperative were only 11 and 15% of predicted rates of absorption. In chronically catheterized rats, > 75% of luminal 3OMG at a concentration of 400 mM was absorbed by active transport. The Km and Vmax of 3OMG active transport corrected for the resistance of the UWL were 11.3 mM and 15.6 mumoles/min, respectively. We conclude that measurements of intestinal glucose absorption performed within 24 h of surgical bowel manipulation greatly underestimate active absorption even if corrections are made to account for the increased resistance of the UWL.
在长期插管的大鼠中确定了手术肠道操作和麻醉对肠道葡萄糖吸收的影响。使用3 - O - 甲基葡萄糖（3OMG）和L - 葡萄糖（D - 葡萄糖的代谢惰性类似物）测量葡萄糖的总吸收速率和被动吸收速率。术后立即及术后4小时的3OMG吸收速率分别比术后6天的吸收速率低86%和62%。术后1天和2天的3OMG吸收速率与术后6天无差异。肠道操作和麻醉未改变L - 葡萄糖的吸收。即使校正了肠道操作后未搅动水层（UWL）增加的阻力，术后立即的肠道葡萄糖总吸收速率和主动吸收速率也仅为预测吸收速率的11%和15%。在长期插管的大鼠中，浓度为400 mM的管腔3OMG中> 75%通过主动转运被吸收。校正UWL阻力后的3OMG主动转运的Km和Vmax分别为11.3 mM和15.6微摩尔/分钟。我们得出结论，即使对UWL增加的阻力进行校正，在手术肠道操作后24小时内进行的肠道葡萄糖吸收测量仍会大大低估主动吸收。
