Bendel C M, St Sauver J, Carlson S, Hostetter M K
Department of Pediatrics, University of Minnesota, Minneapolis 55455, USA.
J Infect Dis. 1995 Jun;171(6):1660-3. doi: 10.1093/infdis/171.6.1660.
Epithelial adhesion and expression of the integrin analog, a putative candidal adhesion, were correlated for 33 clinical and laboratory isolates of Candida albicans, other Candida species, and Saccharomyces cerevisiae. On flow cytometry with saturating concentrations of the monoclonal antibody OKM1, surface fluorescence was highest for C. albicans at 67.8% +/- 1.7% and significantly reduced for Candida tropicalis (32.0% +/- 2.6%), Candida parapsilosis (18.3% +/- 2.4%), Candida glabrata (3.3% +/- 0.8%), Candida lusitaniae (2.9% +/- 1.0%), Candida krusei (0.7% +/- 0.1%), and Saccharomyces cerevisiae (1.7% +/- 0.2%) (P < .006 for all other species vs. C. albicans). Adhesion to a human epithelial cell line was highest for C. albicans at 49.8% +/- 3.5%, lower for C. tropicalis (44.7% +/- 4.6%), and incrementally reduced for all other species (< 25%) (P < .012). The correlation between integrin expression and epithelial adhesion was highly significant (P = .0066; r2 = .8). Surface expression of the integrin analog predicts epithelial adhesion for yeast species isolated in opportunistic infections.
对33株白色念珠菌、其他念珠菌属菌种及酿酒酵母的临床和实验室分离株，检测了上皮黏附情况以及整联蛋白类似物（一种假定的念珠菌黏附分子）的表达，并进行相关性分析。使用饱和浓度的单克隆抗体OKM1进行流式细胞术检测，白色念珠菌的表面荧光最高，为67.8%±1.7%，热带念珠菌（32.0%±2.6%）、近平滑念珠菌（18.3%±2.4%）、光滑念珠菌（3.3%±0.8%）、葡萄牙念珠菌（2.9%±1.0%）、克柔念珠菌（0.7%±0.1%）及酿酒酵母（1.7%±0.2%）的表面荧光显著降低（与白色念珠菌相比，所有其他菌种的P<0.006）。白色念珠菌对人上皮细胞系的黏附率最高，为49.8%±3.5%，热带念珠菌较低（44.7%±4.6%），所有其他菌种的黏附率逐渐降低（<25%）（P<0.012）。整联蛋白表达与上皮黏附之间的相关性非常显著（P = 0.0066；r2 = 0.8）。整联蛋白类似物的表面表达可预测机会性感染中分离出的酵母菌种的上皮黏附情况。
