海参中皂苷的体内和体外生物合成。
In vivo and in vitro biosynthesis of saponins in sea cucumbers.
作者信息
Kerr R G, Chen Z
机构信息
Department of Chemistry, Florida Atlantic University, Boca Raton 33431, USA.
出版信息
J Nat Prod. 1995 Feb;58(2):172-6. doi: 10.1021/np50116a002.
The triterpene precursor of saponins in sea cucumbers has been identified as parkeol [lanost-9(11)-en-3 beta-ol] [1]. Dissection of the sea cucumbers Holothuria floridea and Actinopyga agassize after incubations with radiolabeled parkeol demonstrated that saponin biosynthesis occurs exclusively in the Cuvier gland. This result was corroborated by incubating a cell-free extract of the Cuvier gland with labeled parkeol and observing transformation of the precursor to saponins.
海参中皂苷的三萜前体已被鉴定为帕克醇[羊毛甾-9(11)-烯-3β-醇][1]。在用放射性标记的帕克醇孵育后，解剖佛罗里达海参和阿氏辐肛参，结果表明皂苷生物合成仅发生在居维叶氏器中。通过将居维叶氏器的无细胞提取物与标记的帕克醇一起孵育，并观察前体向皂苷的转化，这一结果得到了证实。
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