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本文引用的文献

1
Passage of Autographa californica nuclear polyhedrosis virus through the midgut epithelium of Spodoptera exigua larvae.苜蓿银纹夜蛾核型多角体病毒通过甜菜夜蛾幼虫中肠上皮的传播
Virology. 1995 Apr 1;208(1):328-35. doi: 10.1006/viro.1995.1156.
2
Programmed cell death in the Drosophila CNS is ecdysone-regulated and coupled with a specific ecdysone receptor isoform.果蝇中枢神经系统中的程序性细胞死亡受蜕皮激素调节,并与一种特定的蜕皮激素受体亚型相关联。
Development. 1993 Dec;119(4):1251-9. doi: 10.1242/dev.119.4.1251.
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Baculovirus gene ME53, which contains a putative zinc finger motif, is one of the major early-transcribed genes.杆状病毒基因ME53含有一个假定的锌指基序,是主要的早期转录基因之一。
J Virol. 1993 Feb;67(2):753-8. doi: 10.1128/JVI.67.2.753-758.1993.
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The complete DNA sequence of Autographa californica nuclear polyhedrosis virus.苜蓿银纹夜蛾核型多角体病毒的完整DNA序列。
Virology. 1994 Aug 1;202(2):586-605. doi: 10.1006/viro.1994.1380.
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Rapid and efficient site-specific mutagenesis without phenotypic selection.无需表型筛选的快速高效位点特异性诱变。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92. doi: 10.1073/pnas.82.2.488.
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A baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyl transferase.一种杆状病毒通过产生蜕皮甾体UDP-葡萄糖基转移酶来阻断昆虫蜕皮。
Science. 1989 Sep 8;245(4922):1110-2. doi: 10.1126/science.2505387.
7
Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector.使用一种基于新型多角体蛋白的杆状病毒表达载体在昆虫细胞中表达花椰菜花叶病毒基因I。
J Gen Virol. 1990 Oct;71 ( Pt 10):2201-9. doi: 10.1099/0022-1317-71-10-2201.
8
Regulation of expression of a baculovirus ecdysteroid UDPglucosyltransferase gene.杆状病毒蜕皮甾体UDP葡萄糖基转移酶基因的表达调控
J Virol. 1990 Mar;64(3):1321-8. doi: 10.1128/JVI.64.3.1321-1328.1990.

杆状病毒蜕皮甾体UDP-葡萄糖基转移酶基因的缺失会导致受感染昆虫中马氏管的早期退化。

Deletion of the baculovirus ecdysteroid UDP-glucosyltransferase gene induces early degeneration of Malpighian tubules in infected insects.

作者信息

Flipsen J T, Mans R M, Kleefsman A W, Knebel-Mörsdorf D, Vlak J M

机构信息

Department of Virology, Agricultural University, Wageningen, The Netherlands.

出版信息

J Virol. 1995 Jul;69(7):4529-32. doi: 10.1128/JVI.69.7.4529-4532.1995.

DOI:10.1128/JVI.69.7.4529-4532.1995
PMID:7769717
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC189199/
Abstract

Deletion of the ecdysteroid UDP-glucosyltransferase gene (egt) from the Autographa californica nuclear polyhedrosis virus (AcNPV) genome increases the speed of killing of this virus (D. R. O'Reilly and L. K. Miller, Bio/Technology 9:1086-1089, 1991). Second-instar Spodoptera exigua larvae are killed more rapidly by the egt deletion mutant of AcNPV than by wild-type AcNPV. Unlike wild-type AcNPV-infected larvae, larvae infected with an egt deletion mutant molt and resume feeding as mock-infected larvae do. Wild-type AcNPV and egt deletion mutant recombinants marked with a lacZ gene were used to study their pathogenesis in insects. Histopathological investigation revealed that early degeneration of the Malpighian tubules, not the molting per se, may be the cause of this increased speed of killing by AcNPV.

摘要

从苜蓿银纹夜蛾核型多角体病毒(AcNPV)基因组中删除蜕皮甾体UDP-葡萄糖基转移酶基因(egt)可提高该病毒的致死速度(D. R. 奥赖利和L. K. 米勒,《生物技术》9:1086 - 1089,1991)。与野生型AcNPV相比,AcNPV的egt缺失突变体杀死二龄甜菜夜蛾幼虫的速度更快。与感染野生型AcNPV的幼虫不同,感染egt缺失突变体的幼虫会蜕皮并恢复进食,就像模拟感染的幼虫一样。用带有lacZ基因标记的野生型AcNPV和egt缺失突变体重组体来研究它们在昆虫中的发病机制。组织病理学研究表明,马氏管的早期退化,而非蜕皮本身,可能是AcNPV致死速度加快的原因。