Barrett J W, Brownwright A J, Primavera M J, Palli S R
Great Lakes Forestry Centre, Canadian Forest Service, Sault Ste. Marie, Ontario, Canada.
J Virol. 1998 Apr;72(4):3377-82. doi: 10.1128/JVI.72.4.3377-3382.1998.
A recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) expressing the green fluorescence protein (GFP) under the control of the AcMNPV polyhedrin promoter was constructed to study the spatial and temporal regulation of baculovirus infection in a permissive host. Larvae that ingested AcMNPV-GFP showed localized expression of GFP in the midgut epithelial cells, as well as hemocytes, at 24 h postinfection. The presence of fluorescence in these tissues indicated not only that the virus was replicating but also that the very late viral proteins were being synthesized. Secondary infection occurred within the tracheal cells throughout the body cavity, confirming earlier reports, and these foci of infection allowed entry of the virus into other tissues, such as the epidermis and the fat body.
构建了一种重组苜蓿银纹夜蛾多粒包埋核型多角体病毒（AcMNPV），其在AcMNPV多角体蛋白启动子的控制下表达绿色荧光蛋白（GFP），以研究杆状病毒在允许宿主中的时空感染调控。摄入AcMNPV-GFP的幼虫在感染后24小时，中肠上皮细胞以及血细胞中出现GFP的局部表达。这些组织中荧光的存在不仅表明病毒在复制，还表明正在合成极晚期病毒蛋白。如先前报道所证实的，气管细胞内发生了全身腔的继发性感染，这些感染灶使病毒能够进入其他组织，如表皮和脂肪体。
