Bikhazi P, Ryan A F
Department of Surgery, University of California, San Diego Medical School, La Jolla 92093, USA.
Laryngoscope. 1995 Jun;105(6):629-34. doi: 10.1288/00005537-199506000-00013.
In both patients and experimental animals, immunoglobulin G (IgG) has been found to dominate acute otitis media with effusion (OME), whereas IgA tends to be present in chronic but not in acute OME. To determine whether local immunoregulation could account for this difference, the expression of cytokines associated with the production of different antibody isotypes was investigated in experimental acute and chronic OME. Mice were systemically immunized and then challenged transtympanically, once to produce an acute OME or once per week for 6 weeks to produce chronic OME. Hybridization with molecular probes for cytokine genes showed that cells producing interleukin-2 (IL-2) and IL-4, but not IL-5, were present during acute OME. In chronic OME, IL-2-positive (IL-2+) and IL-4+ cells were less prevalent, but IL-5+ cells were numerous. These finding support a model by which locally produced IL-2 and IL-4 augment IgG production in acute OME, whereas, IL-5 contributes to increased IgA production in chronic OME.
在患者和实验动物中，均发现免疫球蛋白G（IgG）在分泌性中耳炎（OME）急性期占主导地位，而IgA往往存在于慢性OME中，而非急性OME。为了确定局部免疫调节是否可以解释这种差异，研究人员在实验性急性和慢性OME中研究了与不同抗体同种型产生相关的细胞因子的表达。对小鼠进行全身免疫，然后经鼓膜进行攻击，一次以产生急性OME，或每周一次，持续6周以产生慢性OME。用细胞因子基因的分子探针进行杂交显示，在急性OME期间存在产生白细胞介素-2（IL-2）和IL-4的细胞，但不存在产生IL-5的细胞。在慢性OME中，IL-2阳性（IL-2+）和IL-4+细胞较少见，但IL-5+细胞数量众多。这些发现支持了这样一种模型，即局部产生的IL-2和IL-4在急性OME中增强IgG的产生，而IL-5则有助于慢性OME中IgA产生的增加。
