Oku H, Shudo J, Mimura K, Haratake A, Nagata J, Chinen I
Laboratory of Applied Biochemistry, Faculty of Agriculture, University of The Ryukyus, Okinawa-Ken, Japan.
Lipids. 1995 Feb;30(2):169-72. doi: 10.1007/BF02538271.
A neutral lipid class was isolated by thin-layer chromatography from the skin surface lipids of the hairless mouse. The fraction migrated faster than triglycerides and had a migration rate similar to that of diacyl alkanediols (diester wax). Upon deacylation, however, the long-chain diols were identified as 1-alkylglycerol ethers based on their chromatographic properties and on the mass spectra of their nicotinylidene derivatives. Thus, the skin lipid fraction was identified as 1-O-alkyl-diacylglycerol. The alkyl moieties were all saturated and even-numbered and ranged in chainlength from C16 to C22 with 1-O-hexadecylglycerol amounting to 34% of the total glycerol ether moieties. The fatty acids derived from this lipid fraction were mostly monoenoic with chainlengths ranging from C16 to C24. The major acyl component was eicosenoic acid (20:1) representing 61% of the total fatty acids.
通过薄层层析从无毛小鼠的皮肤表面脂质中分离出一类中性脂质。该组分的迁移速度比甘油三酯快，迁移速率与二酰基链烷二醇（二酯蜡）相似。然而，脱酰基后，根据其色谱性质及其烟碱亚基衍生物的质谱，长链二醇被鉴定为1-烷基甘油醚。因此，皮肤脂质组分被鉴定为1-O-烷基二酰基甘油。烷基部分均为饱和且偶数碳，链长范围为C16至C22，其中1-O-十六烷基甘油占总甘油醚部分的34%。源自该脂质组分的脂肪酸大多为单烯酸，链长范围为C16至C24。主要的酰基成分是二十碳烯酸（20:1），占总脂肪酸的61%。
