Beda M V, Pavliuk A S, Veselova A V, Dement'eva E G, Solov'ev S S, Bart B Ia, Koval'chuk L V
Ter Arkh. 1995;67(3):26-9.
In this study we investigated a phenotype of intact and PHA-stimulated T-lymphocytes from peripheral blood of patients with allergic and nonallergic asthma. Expression of the activation markers (CD25, CD71, HLA-DR) on subsets of T-lymphocytes (CD4+ and CD8+) was determined using double-colour flow cytometry. It was found that nonallergic patients had increased percentages of CD4+CD25+, CD8+CD25+, CD4+HLA-DR+, CD8+HLA-DR+ cells. Allergic patients had a significant increase of CD4+CD25+ cells only. To examine functional significance of these changes mononuclear cells were cultivated with PHA in vitro. Thus, our findings improve our knowledge about asthma pathogenesis and create the grounds for pathogenetically validated immunocorrection.
在本研究中，我们调查了过敏性和非过敏性哮喘患者外周血中完整的和PHA刺激的T淋巴细胞的表型。使用双色流式细胞术测定T淋巴细胞亚群（CD4+和CD8+）上激活标志物（CD25、CD71、HLA-DR）的表达。结果发现，非过敏性患者的CD4+CD25+、CD8+CD25+、CD4+HLA-DR+、CD8+HLA-DR+细胞百分比增加。过敏性患者仅CD4+CD25+细胞显著增加。为了研究这些变化的功能意义，将单核细胞与PHA在体外培养。因此，我们的研究结果增进了我们对哮喘发病机制的了解，并为经病理验证的免疫纠正创造了条件。
