通过脉冲场凝胶电泳对B群脑膜炎奈瑟菌进行流行病学评估。
Epidemiological evaluation of Neisseria meningitidis serogroup B by pulsed-field gel electrophoresis.
作者信息
Yakubu D E, Pennington T H
机构信息
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, UK.
出版信息
FEMS Immunol Med Microbiol. 1995 Feb;10(3-4):185-9. doi: 10.1111/j.1574-695X.1995.tb00032.x.
Genomic DNA from 25 strains of serogroup B Neisseria meningitidis was subjected to pulsed-field gel electrophoresis (PFGE) after digestion with Spe I. N. meningitidis genomic DNA displayed considerable diversity. The diversity we observed among these strains was stable and included isolates from an outbreak that were phenotypically identical. This confirms the value of macrorestriction profiling and PFGE in providing epidemiologically stable strain markers for typing meningococci.
用Spe I酶消化后，对25株B群脑膜炎奈瑟菌的基因组DNA进行脉冲场凝胶电泳（PFGE）分析。脑膜炎奈瑟菌基因组DNA表现出相当大的多样性。我们在这些菌株中观察到的多样性是稳定的，包括来自一次暴发且表型相同的分离株。这证实了宏观限制性酶切图谱分析和PFGE在为脑膜炎球菌分型提供流行病学稳定的菌株标记方面的价值。
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