用于在1天内对大肠杆菌O157:H7及其他革兰氏阴性菌进行分型的快速脉冲场凝胶电泳方案。
Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day.
作者信息
Gautom R K
机构信息
Washington State Department of Health, Public Health Laboratories, Seattle 98155, USA.
出版信息
J Clin Microbiol. 1997 Nov;35(11):2977-80. doi: 10.1128/jcm.35.11.2977-2980.1997.
Abstract
Genomic DNA patterns generated by pulsed-field gel electrophoresis are highly specific for different strains of an organism and have significant value in epidemiologic investigations of infectious-disease outbreaks. Unfortunately, time-consuming and tedious specimen processing is an inherent problem which limits the use of this powerful technology as a real-time epidemic investigational tool. Here, I describe a rapid method to improve the response time and provide specific bacterial strain identification for the typing of Escherichia coli O157:H7 and other gram-negative organisms in a single day.
摘要
脉冲场凝胶电泳产生的基因组DNA图谱对生物体的不同菌株具有高度特异性，在传染病暴发的流行病学调查中具有重要价值。不幸的是，耗时且繁琐的样本处理是一个固有问题，这限制了这项强大技术作为实时疫情调查工具的使用。在此，我描述一种快速方法，可缩短响应时间，并在一天内对大肠杆菌O157:H7及其他革兰氏阴性菌进行分型以提供特定菌株鉴定。
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