Fain P R, Kort E N, Chance P F, Nguyen K, Redd D F, Econs M J, Barker D F
Department of Medical Informatics, University of Utah, Salt Lake City 84108, USA.
Nat Genet. 1995 Mar;9(3):261-6. doi: 10.1038/ng0395-261.
We have constructed a two-dimensional map of 243 markers on the X chromosome. The average distance between markers ordered by two recombinants is 5.4 centiMorgans (cM), which is reduced to 3.2 cM using a less stringent criterion of one recombinant. Map resolution is enhanced by replacing the usual reference marker format with a 2D format, and the two-recombinant rule is more conservative than the lod 3.0 criterion for order. Taken together, crossover mapping and the 2D format produces maps with greater reliability and higher resolution than maps constructed using currently accepted standards. This first high-density crossover-based map of an entire human chromosome provides a model for integrating physical and genetic maps.
我们构建了一张X染色体上243个标记的二维图谱。通过两个重组体排序的标记之间的平均距离为5.4厘摩（cM），使用一个重组体的较宽松标准时该距离降至3.2 cM。通过用二维格式取代通常的参考标记格式可提高图谱分辨率，并且两个重组体规则比用于排序的对数优势比（lod）3.0标准更为保守。综合来看，交叉定位和二维格式所产生的图谱比使用当前公认标准构建的图谱具有更高的可靠性和分辨率。这第一张基于高密度交叉的整个人类染色体图谱为整合物理图谱和遗传图谱提供了一个模型。
