Nair U S, Saeed A M, Muriana P M, Kreisle R A, Barrett B, Sinclair C L, Fleissner M L
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
J Am Vet Med Assoc. 1995 May 1;206(9):1339-44.
In the study reported here, 121 Salmonella enteritidis isolates from human beings and 467 isolates from nonhuman sources were analyzed for plasmid pattern and susceptibility to a panel of antimicrobial agents commonly used as biologic markers. A significant (P < 0.05) number of isolates from nonhuman sources were resistant to beta-lactam antibiotics and tetracycline. Resistance to aminoglycosides, quinolones, and trimethoprim/sulfamethoxazole was uncommon. Of the 588 isolates, 445 (76%) were resistant to 2 or more antimicrobial agents. Sixty of 121 (50%) S enteritidis isolates from human beings were susceptible to all 12 antimicrobial agents, but 425 of 467 (91%) S enteritidis isolates from nonhuman sources expressed resistance to 1 or more of the antimicrobial agents used in the study. Analysis of plasmid profiles revealed that significantly (P < 0.05) more isolates from nonhuman sources had high molecular weight plasmids than did isolates from human beings. Isolates from ceca of chickens were associated with patterns of low molecular weight plasmids. Analysis of results of the study revealed similarities among S enteritidis from human beings and eggs, as determined on the basis of plasmid profiles and antibiotic susceptibility patterns, which may implicate eggs as one of the potential sources for infection of human beings. In addition, periodic monitoring of a substantial number of Salmonella isolates to detect drug resistance may be a prudent practice for use in revising the list of antimicrobial agents commonly used in human beings and other animals.
在本报告的研究中，对121株来自人类的肠炎沙门氏菌分离株和467株来自非人类来源的分离株进行了质粒图谱分析以及对一组常用作生物学标记的抗菌药物的敏感性分析。来自非人类来源的分离株中，有相当数量（P < 0.05）对β-内酰胺类抗生素和四环素耐药。对氨基糖苷类、喹诺酮类和甲氧苄啶/磺胺甲恶唑耐药的情况并不常见。在这588株分离株中，445株（76%）对2种或更多种抗菌药物耐药。121株来自人类的肠炎沙门氏菌分离株中有60株（50%）对所有12种抗菌药物敏感，但467株来自非人类来源的肠炎沙门氏菌分离株中有425株（91%）对研究中使用的1种或更多种抗菌药物耐药。质粒图谱分析显示，来自非人类来源的分离株中具有高分子量质粒的明显（P < 0.05）多于来自人类的分离株。来自鸡盲肠的分离株与低分子量质粒图谱相关。研究结果分析表明，基于质粒图谱和抗生素敏感性图谱确定，来自人类和鸡蛋的肠炎沙门氏菌之间存在相似性，这可能意味着鸡蛋是人类感染的潜在来源之一。此外，定期监测大量沙门氏菌分离株以检测耐药性，可能是用于修订人类和其他动物常用抗菌药物清单的谨慎做法。
