Kostadinov D
Eksp Med Morfol. 1978;17(2):66-71.
Treatment of sheep erythrocytes with 1.5% of formaldehyde alter their immunogenic properties. On the background of immune response to nonfixed erythrocytes, the performed studies showed the following peculiarities in the reaction of mice to fixed erythrocytes: 1) the primary response of animals, in ected with fixed erythrocytes, was rather weak after its determination by the number of rosette forming cells (RFC), antibody forming cells (AFC) in the spleen and by the of hemagglutinins (HA) in serum; 2) in contrast to the primary response the fixed erythrocytes induced a good secondary response, which was considerably higher/according to the number of RFC and titre of HA/in mice sensibilized by nonfixed erytrocytes in advance than in those presensibilized by fixed cells. Therefore the fixed erythrocytes were weaker inducers of immunologic memory than the nonfixed under equal other conditions. 3) In contrast to the nonfixed the fixed erythrocytes did not induce the formation of large amounts of direct AFC during the secondary response as well even then when the animals were presensibilized by nonfixed form of the antigen.
用1.5%的甲醛处理绵羊红细胞会改变其免疫原性。在对未固定红细胞的免疫反应背景下，所进行的研究显示小鼠对固定红细胞的反应具有以下特点：1）通过脾脏中玫瑰花结形成细胞（RFC）、抗体形成细胞（AFC）的数量以及血清中血凝素（HA）的含量来测定，感染固定红细胞的动物的初次反应相当微弱；2）与初次反应相反，固定红细胞诱导出良好的二次反应，根据RFC数量和HA滴度，预先用未固定红细胞致敏的小鼠的二次反应比预先用固定细胞致敏的小鼠的二次反应高得多。因此，在其他条件相同的情况下，固定红细胞诱导免疫记忆的能力比未固定红细胞弱。3）与未固定红细胞相反，即使动物预先用未固定形式的抗原致敏，固定红细胞在二次反应期间也不会诱导大量直接AFC的形成。
