Rakic P, Komuro H
Section of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
J Neurobiol. 1995 Mar;26(3):299-315. doi: 10.1002/neu.480260303.
Confocal laser microscopy, in conjunction with carbocyanine dyes and calcium-sensitive fluorescent indicators, was used in slices and explant cultures of developing cerebellum to study cellular mechanisms underlying a motility of neuronal cell migration. The results indicate that a combination of voltage- and ligand-activated ion channels cooperatively regulates Ca2+ influx into the migrating cells. We suggest that molecules, present in the local cellular milieu, affect cell motility by activating specific ion channels and second messengers that influence polymerization of stiff and contractile cytoskeletal proteins. This early interaction between postmitotic neurons and surrounding cells controls the rate of their movements, sculpts their shapes, establishes their positions, and, therefore, indirectly determines their identities to prior formation of synaptic connections.
共聚焦激光显微镜结合羰花青染料和钙敏感荧光指示剂，用于发育中小脑的切片和外植体培养，以研究神经元细胞迁移运动背后的细胞机制。结果表明，电压激活和配体激活的离子通道共同调节Ca2+流入迁移细胞。我们认为，局部细胞环境中存在的分子通过激活特定的离子通道和第二信使来影响细胞运动，这些离子通道和第二信使会影响僵硬和收缩性细胞骨架蛋白的聚合。有丝分裂后神经元与周围细胞之间的这种早期相互作用控制着它们的运动速度，塑造它们的形状，确定它们的位置，因此，在突触连接预先形成之前间接决定它们的身份。
