[利用单克隆抗体亲和分离分枝杆菌抗原]
[Affinity isolation of mycobacterial antigens on monoclonal antibodies].
作者信息
Chernousova L N, Suranova E D, Kalinina O A, Kulikovskaia N V, Kapina M A, Dem'ianenko N V, Sergeeva L V
出版信息
Probl Tuberk. 1995(2):39-42.
PMID:7777463
Abstract
Affinity chromatography on monoclonal antibodies specific to tuberculous mycobacteria was used to isolate mycobacterial antigens from ultrasonic disintegrate M. tuberculosis H37Rv. Disk-electrophoresis on polyacrylamide gel showed purified antigens to consist of polypeptides with molecular mass 16, 19, 32, 60, 27, 70 and 55 kD. The antigens were tested for enzyme immunoassay of antibodies in the sera from patients with infiltrative pulmonary tuberculosis.
摘要
利用针对结核分枝杆菌的单克隆抗体进行亲和层析，从经超声破碎的结核分枝杆菌H37Rv中分离结核分枝杆菌抗原。聚丙烯酰胺凝胶圆盘电泳显示，纯化后的抗原由分子量为16、19、32、60、27、70和55 kD的多肽组成。对这些抗原进行检测，用于浸润性肺结核患者血清中抗体的酶免疫测定。
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