Penman S
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5251-7. doi: 10.1073/pnas.92.12.5251.
Cell structure, emerging from behind the veil of conventional electron microscopy, appears far more complex than formerly realized. The standard plastic-embedded, ultrathin section can image only what is on the section surface and masks the elaborate networks of the cytoplasm and nucleus. Embedment-free electron microscopy gives clear, high-contrast micrographs of cell structure when combined with removal of obscuring material such as soluble proteins. The resinless ultrathin section is the technique of choice; it is simple and inexpensive, and it uses ordinary electron microscopes. The resulting pictures reveal a world of complex cell structure and function. These images necessarily change our conception of the cytoskeleton, nuclear matrix, mitosis, and the relation of membranes to cytostructure.
从传统电子显微镜的面纱背后浮现出来的细胞结构，似乎比以前意识到的要复杂得多。标准的塑料包埋超薄切片只能成像切片表面的东西，掩盖了细胞质和细胞核的精细网络。无包埋电子显微镜与去除诸如可溶性蛋白质等遮蔽物质相结合时，能给出清晰、高对比度的细胞结构显微照片。无树脂超薄切片是首选技术；它简单且成本低，并且使用普通电子显微镜。所得到的图片揭示了一个复杂的细胞结构和功能的世界。这些图像必然会改变我们对细胞骨架、核基质、有丝分裂以及膜与细胞结构关系的概念。
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