Datta A, Jinks-Robertson S
Department of Biology, Emory University, Atlanta, GA 30322, USA.
Science. 1995 Jun 16;268(5217):1616-9. doi: 10.1126/science.7777859.
Complex processes such as transcription, replication, repair, and recombination require changes in chromatin structure and the interactions of numerous trans-acting factors with DNA sequences, raising the possibility that these processes may be interrelated. Here the effect of transcription on the rate of spontaneous mutation in the yeast Saccharomyces cerevisiae was examined. With the use of a lys2 frameshift allele under the control of a highly inducible promoter, the rate of spontaneous reversion was shown to increase when the mutant gene was highly transcribed. Thus, transcriptionally active DNA and enhanced spontaneous mutation rates are associated in yeast.
诸如转录、复制、修复和重组等复杂过程需要染色质结构的改变以及众多反式作用因子与DNA序列的相互作用，这增加了这些过程可能相互关联的可能性。在此，研究了转录对酿酒酵母自发突变率的影响。利用在高度可诱导启动子控制下的lys2移码等位基因，当突变基因高度转录时，自发回复突变率显示增加。因此，在酵母中，转录活跃的DNA与提高的自发突变率相关联。
