Low-dose challenge by the NMDA receptor antagonist dizocilpine exacerbates the spatial learning deficit in entorhinal cortex-lesioned rats.

We investigated the effects of a bilateral quinolinic acid lesion of the medial entorhinal cortex (EC) on acquisition of a spatial learning task. During reversal of the same task, we challenged the animals by the N-methyl-D-aspartate (NMDA) receptor antagonist dizocilpine (MK-801). Training took postoperatively place in an eight-arm radial maze in which four of eight arms were baited. In the acquisition phase (ten blocks of five trials) of the test, EC-lesioned animals showed a working (WM) and a reference memory (RM) deficit. The WM deficit was prominent at the beginning and fully compensated at the end of the acquisition phase. The RM deficit became more evident during the course of the experiment. In the reversal learning phase (seven blocks of five trials), the formerly unbaited arms were baited and half of the control and lesioned animals were challenged by a low dose of dizocilpine (0.04 mg/kg i.p.) before training. Only lesioned and additionally dizocilpine-treated animals showed a WM deficit that was again compensated and a RM deficit that was stronger at the end of the test. In summary, quinolinic acid lesion of the medial EC induces both WM and RM deficits in rats. The WM deficit is rapidly compensated. Enhancement of these deficits by challenge with dizocilpine in the reversal learning phase suggests that the NMDA receptor system was rendered more sensitive by this type of lesion.