Chung J K, Lee M C, Chung H K, Lim S M, Jang J J, Koh C S
Department of Internal Medicine, Seoul National University, College of Medicine, Korea.
Ann Nucl Med. 1995 Feb;9(1):7-13. doi: 10.1007/BF03165002.
We measured the concentration and distribution of tumor associated antigens, TAG-72 and CEA, in stomach cancer by in vitro quantitative autoradiography (IV-QAR). Frozen sections of 33 specimens were incubated with varying concentrations of 125I-labeled CEA-79.1 and B72.3 antibodies specific for carcinoembryonic antigen (CEA) and tumor-associated glycoprotein-72 (TAG-72), respectively. Computer analysis of specific antibody binding gave maximal binding values which were equal to the concentrations of the antigen or epitope. TAG-72 was detected in 25 specimens, at a concentration ranging from 8.4 to 562.9 pmol/g. CEA was detected in 32 of the 33 specimens and its concentration ranged from 8.8 to 525.3 pmol/g. The distribution of TAG-72 by IV-QAR coincided with that of the tumor cells in 41.4% of the pathologic lesions. The distribution of CEA coincided with the tumor cells in 80.5% of pathologic lesions, nearly twice the TAG-72. The concentration of TAG-72 was significantly higher in mucinous adenocarcinoma and mucin containing adenocarcinomas than other types of adenocarcinomas. There was no significant difference in the concentration of CEA among the pathologic types of stomach cancer. In summary, stomach cancer exhibited wide variations in TAG-72 and CEA expression. CEA expression was more frequent and homogeneous than TAG-72.
我们通过体外定量放射自显影术（IV-QAR）测量了胃癌中肿瘤相关抗原TAG-72和癌胚抗原（CEA）的浓度及分布。将33个标本的冰冻切片分别与不同浓度的125I标记的针对癌胚抗原（CEA）和肿瘤相关糖蛋白-72（TAG-72）的CEA-79.1和B72.3抗体孵育。对特异性抗体结合进行计算机分析得出最大结合值，其等于抗原或表位的浓度。在25个标本中检测到TAG-72，浓度范围为8.4至562.9 pmol/g。在33个标本中的32个检测到CEA，其浓度范围为8.8至525.3 pmol/g。通过IV-QAR检测的TAG-72分布在41.4%的病理病变中与肿瘤细胞的分布一致。CEA的分布在80.5%的病理病变中与肿瘤细胞一致，几乎是TAG-72的两倍。黏液腺癌和含黏液腺癌中TAG-72的浓度显著高于其他类型的腺癌。胃癌不同病理类型中CEA的浓度无显著差异。总之，胃癌中TAG-72和CEA的表达存在广泛差异。CEA的表达比TAG-72更频繁且更均匀。
