Gauchez A S, Riondel J, Jacrot M, Calop J, Favier A
Groupe de Recherche et d'Etudes sur les Pathologies Oxydatives (GREPO), Université J. Fourier, la Tronche, France.
Biol Trace Elem Res. 1995 Jan-Mar;47(1-3):103-9. doi: 10.1007/BF02790106.
Antioxidants and reactive oxygen species are considered to play an important role in experimental in vivo carcinogenesis studies. We attempted in this study to evaluate the repercussions on the antioxidant and lipid peroxide status of the growth of human malignant tumors xenografted into athymic mice. We selected three tumor models: two urothelial carcinomas (bladder tumors stage 3) and one brain tumor (glioblastoma stage 4). All these tumors exhibited a fast growth pattern when xenografted into athymic mice. Tumoral tissue was implanted subcutaneously. After growth establishment each tumor size was measured at regular intervals: every 2 d for bladder tumor and twice a week for glioblastoma. The period of observation was 3 wk for bladder tumors and 5 wk for glioblastoma. At the end of the observation period, all mice were sacrificed; tumoral tissue was taken and blood collected. Superoxide dismutase activity (SOD), glutathione peroxidase activity (GSH-Px), zinc (Zn), selenium (Se), and thiobarbituric acid reactive substances (TBARS) were measured in blood. TBARS alone were measured into tumoral tissue. A modification of the antioxidant blood status was observed in mice xenografted with bladder tumors with decrease in Se status and GSH-Px activities, and increase in TBARS. Such an effect was absent in mice xenografted with glioblastoma. It would appear that an oxygen-mediated stress exists in the animal bearing an implanted tumor compared with the control group, and that tumoral tissue itself is able to induce an oxidative stress into its host. All this leads to a disturbance of the antioxidant defense system.
抗氧化剂和活性氧被认为在体内实验性致癌研究中发挥重要作用。在本研究中，我们试图评估将人类恶性肿瘤异种移植到无胸腺小鼠体内后，肿瘤生长对抗氧化和脂质过氧化物状态的影响。我们选择了三种肿瘤模型：两种尿路上皮癌（3期膀胱肿瘤）和一种脑肿瘤（4期胶质母细胞瘤）。当将所有这些肿瘤异种移植到无胸腺小鼠体内时，它们均呈现快速生长模式。肿瘤组织通过皮下植入。在肿瘤生长稳定后，定期测量每个肿瘤的大小：膀胱肿瘤每2天测量一次，胶质母细胞瘤每周测量两次。膀胱肿瘤的观察期为3周，胶质母细胞瘤的观察期为5周。在观察期结束时，处死所有小鼠；采集肿瘤组织和血液。测量血液中的超氧化物歧化酶活性（SOD）、谷胱甘肽过氧化物酶活性（GSH-Px）、锌（Zn）、硒（Se）和硫代巴比妥酸反应性物质（TBARS）。仅在肿瘤组织中测量TBARS。在移植膀胱肿瘤的小鼠中观察到抗氧化血液状态的改变，硒状态和GSH-Px活性降低，TBARS升高。在移植胶质母细胞瘤的小鼠中未观察到这种效应。与对照组相比，似乎植入肿瘤的动物存在氧介导的应激，并且肿瘤组织本身能够在其宿主中诱导氧化应激。所有这些导致抗氧化防御系统的紊乱。
