Calomme M, Hu J, Van den Branden K, Vanden Berghe D A
Laboratory of Microbiology, Faculty of Medicine, University of Antwerp, Belgium.
Biol Trace Elem Res. 1995 Jan-Mar;47(1-3):379-83. doi: 10.1007/BF02790140.
Lactic acid bacteria are nonpathogenic bacteria commonly used in food processing. An evaluation was made of the capacity to concentrate selenium in species of Lactobacillus. A selenium concentration of 1 microgram/mL in the culture medium yielded in a bacterial content of 400 micrograms/g dry biomass. Dialysis and TCA precipitation experiments of a native intracellular extract proved that at least 80% of the total selenium is associated with organic molecules. Seleno-cysteine was identified as the only seleno-amino acid present in the intracellular selenoproteins. This study shows that species of the lactic acid bacteria are able to concentrate selenium intracellular as seleno-cysteine, which could be applied in supplementation studies.