采用高pH值阴离子交换色谱-脉冲安培检测法分析胆汁酸及其结合物。

Analysis of bile acids and their conjugates using high-pH anion-exchange chromatography with pulsed amperometric detection.

作者信息

Chaplin M F

机构信息

School of Applied Science, South Bank University, London, UK.

出版信息

J Chromatogr B Biomed Appl. 1995 Feb 17;664(2):431-4. doi: 10.1016/0378-4347(94)00488-q.

DOI:10.1016/0378-4347(94)00488-q

PMID:7780598

Abstract

Bile acids and their conjugated forms may be separated by anion-exchange chromatography in alkaline media (0.9 M sodium acetate, 0.1 M sodium hydroxide, 15% v/v acetonitrile) on a CarboPac PA-100 column. The effluent was monitored at high sensitivity, with detection limits of less than 10 microM, using a pulsed amperometric detector. Free bile acids and their glyco- and tauro-conjugated forms were separated and detected within 40 min under isocratic conditions.

摘要

胆汁酸及其结合形式可在碱性介质（0.9 M醋酸钠、0.1 M氢氧化钠、15% v/v乙腈）中，于CarboPac PA - 100柱上通过阴离子交换色谱法进行分离。使用脉冲安培检测器对流出物进行高灵敏度监测，检测限低于10微摩尔。在等度条件下，游离胆汁酸及其糖基和牛磺酸结合形式在40分钟内被分离并检测出来。