Halbhuber K J, Unger J, Fröber R, Geyer G
Folia Haematol Int Mag Klin Morphol Blutforsch. 1978;105(1):102-8.
The volume of human red blood cells (RBC) was evaluated by means of the centrifugation method (hematocrit) and 131-J-labelled human serum albumin, respectively. Both of the methods yielded an identical volume of about 107 micron3 of the single RBC, provided the evaluation was performed in autologous plasma. Contrary to the 131-J-albumin method the results of which were found independent of various pretreatments of RBC, the centrifugation hematocrits of RBC previously washed with PBS and resuspended in PBS or saline protein media resulted in a mean cell volume of about 86 micron3. The decrease of the cell volume was associated with an efflux of K+ ions. If the RBC are centrifuged at 800 g instead of 15000 g, their volume will remain unchanged. The assessment of cytodeformability has shown, that RBC in PBS by loss of cell volume could enter a 2.3 micron micropipette completely. RBC in plasma, though traversing a 2.9 micron micropipette were incapable of entering a 2.3 micron channel completely. With pressures ranging from 300 to 350 mm H2O the processes of these cells undergo microspherulation.
分别采用离心法（血细胞比容）和131-J标记的人血清白蛋白对人红细胞（RBC）的体积进行评估。如果在自体血浆中进行评估，这两种方法得出的单个红细胞体积均约为107立方微米。与131-J白蛋白法不同，后者的结果与红细胞的各种预处理无关，而用PBS洗涤并重新悬浮于PBS或盐蛋白介质中的红细胞的离心血细胞比容得出的平均细胞体积约为86立方微米。细胞体积的减小与钾离子外流有关。如果红细胞以800 g而不是15000 g的速度离心，其体积将保持不变。细胞变形性评估表明，PBS中的红细胞因细胞体积减小能够完全进入2.3微米的微量移液器。血浆中的红细胞虽然能穿过2.9微米的微量移液器，但无法完全进入2.3微米的通道。在300至350毫米水柱的压力下，这些细胞会发生微球化。
