Isobe Y, Nakajima K, Nishino H
Department of Physiology, Nagoya City University Medical School, Japan.
Brain Res Dev Brain Res. 1995 Mar 16;85(1):58-63. doi: 10.1016/0165-3806(94)00192-3.
To investigate the development of Arg-vasopressin (AVP) content and its diurnal rhythm in the suprachiasmatic nucleus (SCN), AVP was measured by enzyme immunoassay in the rat pup SCN punched out from tissue slices obtained at postnatal day (PD) 1, 5, 10, 12 and 20 from animals maintained under a light-dark cycle (LD). The AVP levels, measured at a restricted time of day, increased from PD1 reaching the adult level at PD10-12. Diurnal rhythm similar to that in adults was evident at PD10-12 under the LD conditions used. The peak value of AVP was observed at the earlier light period, and its trough occurred at the end of the light period. The circadian rhythm remained for 7 days under constant dark (DD) or constant light (LL) conditions. These results indicate that the AVP, one of the output signals from the SCN, starts to oscillate after PD10-12, and shows a free-running rhythm during the nursing period.
为研究视交叉上核（SCN）中精氨酸加压素（AVP）含量的发育及其昼夜节律，采用酶免疫分析法，对在明暗周期（LD）下饲养的动物于出生后第1、5、10、12和20天从组织切片中取出的大鼠幼崽SCN进行AVP测定。在一天中的特定时间测量的AVP水平从出生后第1天开始升高，在出生后第10 - 12天达到成年水平。在所采用的LD条件下，出生后第10 - 12天出现了与成年动物相似的昼夜节律。AVP的峰值出现在较早的光照期，其谷值出现在光照期结束时。在持续黑暗（DD）或持续光照（LL）条件下，昼夜节律持续7天。这些结果表明，作为SCN输出信号之一的AVP在出生后第10 - 12天开始振荡，并在哺乳期表现出自主节律。
