Plusczyk T, Bauer M, Marzi I, Harbauer G, Feifel G
Department of General Surgery, University of Saarland, Homburg/Saar, Germany.
Dig Dis Sci. 1995 Jun;40(6):1199-206. doi: 10.1007/BF02065524.
Using epifluorescent microscopy, we investigated the dynamic changes in pancreatic microcirculation in vivo after bolus administration of secretin (SEC) (0.1-10.0 micrograms/100 g body wt) and cholecystokinin-octapeptide (CCK-8) (0.005-1.2 micrograms/100 g body wt) in pentobarbital-anesthetized rats. Pancreatic capillary red cell velocity as a monitor for pancreatic capillary blood flow was measured in 1-min intervals from 2 min prior to 8 min following bolus infusion of SEC or CCK-8. Physiological concentrations of SEC did not increase pancreatic capillary blood flow. However, pharmacological SEC concentrations induced a dose-dependent increase in pancreatic capillary blood flow (to 162 +/- 19% of baseline; P < 0.05), due to an increase in blood flow velocity (to 153 +/- 18% of baseline; P < 0.05). In contrast, bolus administration of physiological CCK-8 concentrations, which have been proven to stimulate enzyme secretion, induced a transient and dose-dependent increase in pancreatic capillary blood flow (to 235 +/- 24% of baseline; P < 0.05), due to an increase in blood flow velocity (to 184 +/- 13% of baseline; P < 0.05) and capillary diameters (+0.63 +/- 0.15 micron; P < 0.05).
我们采用落射荧光显微镜，研究了在戊巴比妥麻醉的大鼠中，静脉推注促胰液素（SEC）（0.1 - 10.0微克/100克体重）和八肽胆囊收缩素（CCK - 8）（0.005 - 1.2微克/100克体重）后胰腺微循环的动态变化。在静脉推注SEC或CCK - 8前2分钟至推注后8分钟期间，每隔1分钟测量一次胰腺毛细血管红细胞速度，以此作为胰腺毛细血管血流的监测指标。生理浓度的SEC并未增加胰腺毛细血管血流。然而，药理浓度的SEC可引起胰腺毛细血管血流呈剂量依赖性增加（达到基线的162±19%；P < 0.05），这是由于血流速度增加（达到基线的153±18%；P < 0.05）。相比之下，已证实可刺激酶分泌的生理浓度CCK - 8静脉推注后，可引起胰腺毛细血管血流短暂且呈剂量依赖性增加（达到基线的235±24%；P < 0.05），这是由于血流速度增加（达到基线的184±13%；P < 0.05）以及毛细血管直径增加（+0.63±0.15微米；P < 0.05）。
