牛疱疹病毒4保守γ疱疹病毒基因块以外基因组区域的分析

Analysis of bovine herpesvirus 4 genomic regions located outside the conserved gammaherpesvirus gene blocks.

作者信息

Lomonte P, Bublot M, van Santen V, Keil G M, Pastoret P P, Thiry E

机构信息

Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liege, Belgium.

出版信息

J Gen Virol. 1995 Jul;76 ( Pt 7):1835-41. doi: 10.1099/0022-1317-76-7-1835.

DOI:10.1099/0022-1317-76-7-1835

PMID:9049390

Abstract

Bovine herpesvirus 4 (BHV-4) DNA sequences located outside the gene blocks conserved among the gammaherpesviruses BHV-4, herpesvirus saimiri (HVS) and Epstein-Barr virus (EBV) were analysed. Twelve potential open reading frames (ORFs) were found. Protein database comparisons showed that no ORF translation products were similar to proteins encoded by alpha- or betaherpesviruses. Nevertheless, six of the ORFs were homologous in amino acid sequences to proteins encoded by HVS but apparently not to those encoded by EBV. Furthermore, the location and orientation of these six ORFs in the BHV-4 genome were similar to the corresponding ORFs in the HVS genome. No genes homologous to known cellular genes were found in the BHV-4 genome; this feature is the major difference between the BHV-4 and HVS genomes with regards to the overall gene content.

摘要

对牛疱疹病毒4（BHV - 4）中位于γ疱疹病毒BHV - 4、猴疱疹病毒（HVS）和爱泼斯坦 - 巴尔病毒（EBV）保守基因块之外的DNA序列进行了分析。发现了12个潜在的开放阅读框（ORF）。蛋白质数据库比较显示，没有ORF翻译产物与α或β疱疹病毒编码的蛋白质相似。然而，其中6个ORF在氨基酸序列上与HVS编码的蛋白质同源，但显然与EBV编码的蛋白质不同源。此外，这6个ORF在BHV - 4基因组中的位置和方向与HVS基因组中的相应ORF相似。在BHV - 4基因组中未发现与已知细胞基因同源的基因；就整体基因内容而言，这一特征是BHV - 4和HVS基因组之间的主要区别。