Tabuchi K, Kirsch W M, Nakane P K
J Neurol Sci. 1976 May;28(1):65-76. doi: 10.1016/0022-510x(76)90048-4.
This report describes the first fine structural locations of S-100 protein in the hamster cerebellum. Innovations in tissue fixation as well as tracer preparation enable localization of S-100 protein to several critical cellular and subcellular sites. Certain neuronal elements, Purkinje cells, contain S-100 protein in both cytoplasm and nucleus. Specific membrane structures including plasma membranes and the nuclear membranes of Purkinje cells are strikingly positive. Localization of S-100 protein to the pericapillary astrocytic endfeet as well as regions of the synapse are of particular biological interest. The clear nuclear localization of the molecule suggests a role in the genetic expression of the developing mammalian brain.
本报告描述了S-100蛋白在仓鼠小脑中的首个精细结构定位。组织固定以及示踪剂制备方面的创新使得S-100蛋白能够定位到几个关键的细胞和亚细胞位点。某些神经元成分，即浦肯野细胞，在细胞质和细胞核中均含有S-100蛋白。包括浦肯野细胞质膜和核膜在内的特定膜结构呈现出显著的阳性反应。S-100蛋白在毛细血管周围星形胶质细胞终足以及突触区域的定位具有特别的生物学意义。该分子在细胞核中的明确定位表明其在发育中的哺乳动物大脑的基因表达中发挥作用。
