Yamamoto T, Moriwaki Y, Takahashi S, Suda M, Higashino K
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
Metabolism. 1995 Jun;44(6):779-85. doi: 10.1016/0026-0495(95)90192-2.
In the present study, we investigated whether ethanol inhibits the activity of xanthine dehydrogenase. Ethanol and/or inosine were administered to normal subjects, and plasma concentration and urinary excretion of purine bases were measured together with blood concentrations of lactic acid and pyruvic acid. In addition, ethanol and pyrazinamide were administered to these subjects, and plasma concentration and urinary excretion of pyrazinamide and its major metabolites were measured. Increases in plasma concentration and urinary excretion of xanthine induced by a combination of ethanol and inosine were greater than the sums of increases induced separately by ethanol and inosine, although increases in plasma concentration and urinary excretion of uric acid induced by the combination of ethanol and inosine were not different from the sums of increases induced separately by ethanol and inosine. Ethanol increased the ratio of blood lactic acid to blood pyruvic acid and decreased plasma concentration and urinary excretion of 5-hydroxypyrazinamide and 5-hydroxypyrazinoic acid. These results suggest that ethanol inhibits xanthine dehydrogenase presumably by an ethanol-induced increase in the cytosolic concentration of NADH in the liver.
在本研究中，我们调查了乙醇是否会抑制黄嘌呤脱氢酶的活性。将乙醇和/或肌苷给予正常受试者，并测量嘌呤碱的血浆浓度和尿排泄量，同时测量血液中乳酸和丙酮酸的浓度。此外，将乙醇和吡嗪酰胺给予这些受试者，并测量吡嗪酰胺及其主要代谢产物的血浆浓度和尿排泄量。乙醇和肌苷联合诱导的黄嘌呤血浆浓度和尿排泄量的增加大于乙醇和肌苷分别诱导的增加量之和，尽管乙醇和肌苷联合诱导的尿酸血浆浓度和尿排泄量的增加与乙醇和肌苷分别诱导的增加量之和没有差异。乙醇增加了血液中乳酸与丙酮酸的比值，并降低了5-羟基吡嗪酰胺和5-羟基吡嗪酸的血浆浓度和尿排泄量。这些结果表明，乙醇可能通过乙醇诱导的肝脏细胞溶质中NADH浓度增加来抑制黄嘌呤脱氢酶。
