与DNA复合的偶数跳节同源域的纯化、结晶及初步X射线衍射分析。

Purification, crystallization, and preliminary X-ray diffraction analysis of even-skipped homeodomain complexed to DNA.

作者信息

Hirsch J A, Aggarwal A K

机构信息

Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.

出版信息

Proteins. 1995 Mar;21(3):268-71. doi: 10.1002/prot.340210311.

DOI:10.1002/prot.340210311

PMID:7784431

Abstract

Embryonic development in metazoa, to a significant extent, is directed by genes which contain a conserved sequence motif named the homeobox. This sequence encodes a polypeptide called the homeodomain which has sequence specific DNA-binding activity. We report the purification, crystallization, and preliminary diffraction analysis of the Drosophila Even-skipped homeodomain (Eve HD) bound to two different oligonucleotides. Crystals of Eve HD complexed with an AT-rich sequence belong to space group P2(1), a = 34.06, b = 61.61, c = 39.99 Angstrom, beta = 90.0 degrees. These crystals diffract to at least 2.0 Angstrom and both native and derivative data sets have been collected. Crystals of Eve HD complexed with a GC-rich sequence belong to space group P6(3), a = b = 124.52, c = 66.78 Angstrom and diffract to 3.5 Angstrom resolution. A native data set has been collected.

摘要

后生动物的胚胎发育在很大程度上由含有一个名为同源异型框的保守序列基序的基因所指导。该序列编码一种名为同源异型结构域的多肽，其具有序列特异性DNA结合活性。我们报告了与两种不同寡核苷酸结合的果蝇偶数跳动同源异型结构域（Eve HD）的纯化、结晶及初步衍射分析。与富含AT序列复合的Eve HD晶体属于空间群P2(1)，a = 34.06，b = 61.61，c = 39.99埃，β = 90.0°。这些晶体衍射至至少2.0埃，并且已经收集了天然和衍生数据集。与富含GC序列复合的Eve HD晶体属于空间群P6(3)，a = b = 124.52，c = 66.78埃，衍射至3.5埃分辨率。已经收集了一个天然数据集。