Vigre H, Ersbøll A K, Sørensen V
Department of Science and Animal Health, Royal Veterinary and Agricultural University, Grønnegårdsvej 8, DK-1870 Frederiksberg C, Denmark.
J Vet Med B Infect Dis Vet Public Health. 2003 Nov;50(9):430-5. doi: 10.1046/j.0931-1793.2003.00700.x.
The main objective of this study was to estimate the decay of acquired colostral antibodies to Actinobacillus pleuropneumoniae serotype 2 in pigs. Data were obtained from pigs in an isolated cohort of 47 pigs born to five sows seropositive to A. pleuropneumoniae serotype 2. The pigs were examined serologically at 18 different times from birth until an age of about 22 weeks, using an A. pleuropneumoniae serotype 2-specific blocking enzyme-linked immunosorbent assay. Antibody concentration was expressed as an OD% derived from the optical density of the sample and the median from eight wells without serum on the same plate. A non-linear mixed model assuming a constant rate of decay (half-life) was specified and fitted to the serological data. To estimate the between-pig variability of different components, between-pig random effects of each component of the model were estimated. The estimated average half-life of acquired colostral antibodies was approximately 2 weeks, but there was a considerable variation between pigs (half-life ranged from 1-3 weeks). The duration until acquired colostral antibodies were no longer detectable ranged from 2 weeks to 2 months postpartum among the pigs in the study, mainly depending on the initial level of acquired colostral antibodies to A. pleuropneumoniae serotype 2.
本研究的主要目的是评估猪体内获得性初乳抗体对胸膜肺炎放线杆菌2型的衰减情况。数据来自一个隔离猪群中的猪，该猪群有47头猪，由5头对胸膜肺炎放线杆菌2型血清学阳性的母猪所生。从出生到约22周龄期间，在18个不同时间点对这些猪进行血清学检测，采用胸膜肺炎放线杆菌2型特异性阻断酶联免疫吸附测定法。抗体浓度以样本光密度衍生的OD%表示，并以同一板上八个无血清孔的中位数表示。指定了一个假设衰减率恒定（半衰期）的非线性混合模型，并将其拟合到血清学数据。为了估计不同组分在猪之间的变异性，估计了模型各组分在猪之间的随机效应。获得性初乳抗体的估计平均半衰期约为2周，但猪之间存在相当大的差异（半衰期范围为1 - 3周）。在本研究的猪中，可以检测到获得性初乳抗体的持续时间从产后2周到2个月不等，主要取决于获得性初乳抗体对胸膜肺炎放线杆菌2型的初始水平。
