Pfalzgraf A, Steinhart H, Frigg M
Institut für Biochemie und Lebensmittelchemie, Universität Hamburg, Germany.
Z Lebensm Unters Forsch. 1995 Mar;200(3):190-3. doi: 10.1007/BF01190492.
A fast, sensitive and reproducible method for the analysis of alpha-tocopherol in pork tissues is presented. It combines saponification of the tissue and alpha-tocopherol extraction in a single vessel, followed by HPLC separation and fluorescence detection. Added alpha-tocopherol was recovered quantitatively. The reduction of lipid peroxides with potassium iodide before the saponification step did not alter the amounts of alpha-tocopherol detected. Membrane-bound alpha-tocopherol was not oxidized by lipid peroxides during the procedure. The coefficient of variation of alpha-tocopherol analysed using this method was +/- 4.2% for muscle and +/- 2.5% for adipose tissues.
本文提出了一种快速、灵敏且可重复的猪肉组织中α-生育酚分析方法。该方法将组织皂化和α-生育酚提取合并在一个容器中进行，随后进行高效液相色谱分离和荧光检测。添加的α-生育酚能够被定量回收。在皂化步骤之前用碘化钾还原脂质过氧化物不会改变所检测到的α-生育酚含量。在该过程中，膜结合的α-生育酚不会被脂质过氧化物氧化。使用此方法分析α-生育酚时，肌肉的变异系数为±4.2%，脂肪组织的变异系数为±2.5%。
