Liu Q, Scheller K K, Schaefer D M
Department of Meat and Animal Science, University of Wisconsin-Madison 53706-1284, USA.
J Anim Sci. 1996 Oct;74(10):2406-10. doi: 10.2527/1996.74102406x.
A simplified method for the determination of alpha-tocopherol concentration in beef muscle was developed and evaluated. The method consists of a saponification step applied to 1-g samples of intact, fresh muscle, followed by a single isooctane extraction of the saponified samples. alpha-Tocopherol in the extract was separated by normal phase chromatography and quantified by fluorescence detection. A single extraction with the simplified method accounted for 95% of the total muscle alpha-tocopherol concentration obtained by two extractions with the Arnold et al. (J. Food Sci. 58:28, 1993) method. Recovery of added alpha-tocopherol standard after two extractions of a saponified muscle sample was 91% for the simplified method, which was not different (alpha = .78) from recovery using the Arnold method, and the efficiency of the single extraction in the simplified method was 89%. The coefficients of variation for the simplified and Arnold methods were both 3.1%. This method should permit the duplicate analysis of 100 fresh muscle samples within 24 h.
开发并评估了一种测定牛肉肌肉中α-生育酚浓度的简化方法。该方法包括对1克完整新鲜肌肉样品进行皂化步骤，然后对皂化后的样品用异辛烷进行单次萃取。萃取物中的α-生育酚通过正相色谱法分离，并用荧光检测法定量。用简化方法进行单次萃取得到的肌肉α-生育酚总浓度占用阿诺德等人（《食品科学杂志》58:28，1993年）方法进行两次萃取所得浓度的95%。对于简化方法，皂化后的肌肉样品经过两次萃取后添加的α-生育酚标准品的回收率为91%，与使用阿诺德方法的回收率无差异（α = 0.78），简化方法中单次萃取的效率为89%。简化方法和阿诺德方法的变异系数均为3.1%。该方法应能在24小时内对100个新鲜肌肉样品进行重复分析。
