Zuijderhoudt F M, Dorresteijn-de Bok J, Te Velde K
Department of Clinical Chemistry, Stichting Deventer Ziekenhuizen, Deventer, The Netherlands.
Ann Clin Biochem. 1995 Mar;32 ( Pt 2):186-9. doi: 10.1177/000456329503200209.
We compared a spectrophotometric screening test for urine porphyrin concentration with a high performance liquid chromatography (HPLC) method. The screening test gave lower values than those obtained by HPLC, but the overall correlation was good. Occasionally, spectrophotometry failed to detect porphyrins in the urine which were detected by HPLC. The type of porphyria had no influence on the efficacy of the screening method. Receiver operating characteristic plot analysis of the screening test led to a cut-off value of 110 nmol/24 h with a sensitivity of 96% and a specificity of 86%. We conclude that the spectrophotometric screening method is useful for detection of increased total urine porphyrin concentration.
我们将一种用于检测尿卟啉浓度的分光光度筛查试验与高效液相色谱（HPLC）法进行了比较。筛查试验得出的值低于HPLC法测得的值，但总体相关性良好。偶尔，分光光度法未能检测出HPLC法所检测到的尿中的卟啉。卟啉病的类型对筛查方法的效果没有影响。对筛查试验进行的受试者工作特征曲线分析得出临界值为110 nmol/24 h，灵敏度为96%，特异性为86%。我们得出结论，分光光度筛查方法有助于检测尿中总卟啉浓度的升高。
