Zuijderhoudt F M, Dorresteijn-de Bok J
Department of Clinical Chemistry, Deventer Ziekenhuis, The Netherlands.
Ann Clin Biochem. 1998 May;35 ( Pt 3):418-21. doi: 10.1177/000456329803500312.
We compared two screening methods for increased urine porphyrin concentration and compared the results with a high-performance liquid chromatography (HPLC) method. The screening methods were the Bio-Rad (Porphyrin) Column Test and a simple spectrophotometric method. Results were obtained for urines with three different porphyrin patterns. Both screening methods were easy to perform. The accuracy and precision of the spectrophotometric method were both slightly better than that of the Bio-Rad Column Test. Recovery measurements in samples with different porphyrin patterns varied between 73% and 59% (n = 12) for the spectrophotometric method and between 82% and 116% (n = 12) for the Bio-Rad Column Test as compared to HPLC. Between batch precision measurements revealed coefficients of variation for spectrophotometric and Bio-Rad methods for 2%-4% and 4%-10%, respectively. The recovery of the porphyrins illustrates the Bio-Rad Column Test to be more susceptible to variation in urine porphyria composition. Both methods will show satisfactory results in cases of overt porphyria because of the high urine porphyrin concentration.
我们比较了两种用于检测尿卟啉浓度升高的筛查方法，并将结果与高效液相色谱（HPLC）法进行了比较。筛查方法为Bio-Rad（卟啉）柱试验和一种简单的分光光度法。对具有三种不同卟啉模式的尿液进行了检测。两种筛查方法操作都很简便。分光光度法的准确度和精密度均略优于Bio-Rad柱试验。与HPLC相比，分光光度法在不同卟啉模式样品中的回收率在73%至59%之间（n = 12），Bio-Rad柱试验的回收率在82%至116%之间（n = 12）。批间精密度测量显示，分光光度法和Bio-Rad法的变异系数分别为2% - 4%和4% - 10%。卟啉的回收率表明Bio-Rad柱试验更容易受到尿卟啉组成变化的影响。由于尿卟啉浓度较高，两种方法在明显卟啉症病例中均会显示出满意的结果。
