Pekarsky A, Rust P F, Varn E, Mathur R S, Mathur S
College of Charleston, South Carolina, USA.
Arch Androl. 1995 Mar-Apr;34(2):77-82. doi: 10.3109/01485019508987834.
A previous study from this laboratory showed that nicotine in vitro has deleterious effects on sperm motion characteristics. This study was conducted to evaluate the effects of nicotine on the ability of human spermatozoa to attach and penetrate zona-free hamster eggs. Spermatozoa from fertile donors, washed free of seminal plasma, were incubated with medium (control) and 0.1, 1, 5, and 10 mM concentrations of nicotine (concentrations estimated to approximate residual concentrations of nicotine in the testes of heavy smokers) for 18 h at 37 degrees C in a humid 5% carbon dioxide incubator. The sperm preparations were then mixed with enzymatically denuded hamster eggs and incubated for 3 h at 37 degrees C. The oocytes were examined by phase-contrast microscopy to enumerate the rates of sperm attachment and penetration. The data were analyzed by a paired t test and repeated measures analysis of variance using the arcsine transformation of the percentages. The percentages of eggs with attached spermatozoa significantly declined in a dose-dependent manner, the highest inhibition being at 10 mM (F = 24). The rate of sperm penetration was even more significantly decreased with the increase in nicotine concentrations in the following order: 10 mM (F = 56) > 5 mM (F = 30) > 1 mM (F = 44) > 0.1 mM (F = 12). Nicotine concentrations of 0.1 mM and above negatively affected sperm penetration of zona-free hamster eggs.
该实验室之前的一项研究表明，尼古丁在体外对精子运动特性具有有害影响。本研究旨在评估尼古丁对人类精子附着并穿透去透明带仓鼠卵能力的影响。来自可育供体的精子，经洗涤去除精浆后，在湿度为5%二氧化碳的培养箱中于37℃下与培养基（对照）以及0.1、1、5和10 mM浓度的尼古丁（估计这些浓度近似重度吸烟者睾丸中尼古丁的残留浓度）孵育18小时。然后将精子制剂与经酶法去除透明带的仓鼠卵混合，并在37℃下孵育3小时。通过相差显微镜检查卵母细胞，以计算精子附着和穿透的比率。使用百分比的反正弦转换，通过配对t检验和重复测量方差分析对数据进行分析。附着有精子的卵的百分比以剂量依赖性方式显著下降，最高抑制作用出现在10 mM时（F = 24）。随着尼古丁浓度增加，精子穿透率下降更为显著，顺序如下：10 mM（F = 56）> 5 mM（F = 30）> 1 mM（F = 44）> 0.1 mM（F = 12）。0.1 mM及以上浓度的尼古丁对精子穿透去透明带仓鼠卵产生负面影响。
