Sterzik K, Strehler E, Abt M, Rosenbusch B
Department of Gynecology and Obstetrics, University of Ulm, Germany.
J Urol. 1994 Jun;151(6):1712-4. doi: 10.1016/s0022-5347(17)35350-8.
We investigated the effect of an in vitro kallikrein treatment of human spermatozoa on their ability to penetrate zona-free hamster eggs. Sixty-four infertility patients were classified into four groups (normo-, oligo-, astheno-, and oligoasthenozoospermia) in order to find out which men would benefit most from this procedure. An aliquot of each ejaculate was diluted with Ham's F-10 medium supplemented with human serum albumin and kept at 22C for 1 hour. Another aliquot was incubated with kallikrein (5 KU/ml.) for one hour at 22C. These sperm suspensions were then washed twice, subjected to a swim-up preparation, and subsequently incubated at 37C for 2 hours. The mean penetration rates of kallikrein-treated samples were significantly higher than those of the corresponding control assays in all but the oligoasthenozoospermia group. Our results suggest a direct proteolytic action of kallikrein because the stimulation of penetration into hamster eggs was not correlated with the amount of constituents of the kallikrein-kinin system (kinins, kininogen, kininase) in seminal plasma.
我们研究了用体外激肽释放酶处理人类精子对其穿透去透明带仓鼠卵能力的影响。64名不育患者被分为四组（正常精子症、少精子症、弱精子症和少弱精子症），以便找出哪些男性将从该程序中获益最多。每份精液标本的一部分用添加了人血清白蛋白的哈姆氏F-10培养基稀释，并在22℃下保存1小时。另一部分在22℃下与激肽释放酶（5KU/ml）孵育1小时。然后将这些精子悬液洗涤两次，进行上游制备，随后在37℃下孵育2小时。除少弱精子症组外，激肽释放酶处理样本的平均穿透率均显著高于相应的对照试验。我们的结果提示激肽释放酶有直接的蛋白水解作用，因为其对穿透仓鼠卵的刺激与精浆中激肽释放酶-激肽系统的成分（激肽、激肽原、激肽酶）的量无关。
