Campos F G, Mucerino D R, Waitzberg D L, Logulo A F, el Ibrahim R, Nadalin W, Habr-Gama A
Departamento de Cirurgia da Faculdade de Medicina da Universidade de S. Paulo.
Rev Assoc Med Bras (1992). 1994 Jul-Sep;40(3):143-9.
Radiotherapy plays nowadays an important role in malignancies treatment. However, collateral effects and severe complications owing to cellular damage of peritumoral tissues may occur. Different nutritional resources have been recently indicated to achieve intestinal protection during cancer irradiation. PURPOSE--The aim of this study was to set the role of glutamine and elemental diets in acute actinic enteritis prevention. METHOD--Sixty-five adult male Wistar rats with average weight of 200g were maintained in individual metabolic cages; daily body weight and food ingestion were carefully monitored. The animals were randomized into three groups and fed isocaloric and isonitrogenous diets: 1) CRt-polymeric-casein diet; 2) GRt-polymeric-casein diet supplemented with 2% glutamine and 3) ERt-elemental diet supplemented with 2% glutamine. After an adaptation period (seven days), all rats received abdominal radiation in five daily doses of 300cGy. Four days after the rats were operated on to resect the small intestine and colon for histological evaluation. RESULTS--Small intestine histological data in ERt and GRt rats were better than CRt rats, by preserving mucosal cellularity and increasing mitosis number and villi length. Simultaneously, ERt group had greater number of rats with normal villus-crypt relation than CRt or GRt groups. Large intestine histological data showed that the average crypts length in ERt and GRt rats were greater than in CRt ones. CONCLUSION--Glutamine-supplemented polymeric or elemental diets given to rats before, during and after abdominal radiotherapy showed protective effects against radiation injury, by supporting mucosal structure and recovery.
如今，放射疗法在恶性肿瘤治疗中发挥着重要作用。然而，由于肿瘤周围组织的细胞损伤，可能会出现副作用和严重并发症。最近有研究表明，不同的营养资源可在癌症放疗期间实现肠道保护。目的——本研究旨在确定谷氨酰胺和要素饮食在预防急性放射性肠炎中的作用。方法——65只平均体重为200g的成年雄性Wistar大鼠饲养在单独的代谢笼中；每天仔细监测体重和食物摄入量。将动物随机分为三组，给予等热量和等氮饮食：1）CRt-聚合酪蛋白饮食；2）GRt-聚合酪蛋白饮食补充2%谷氨酰胺；3）ERt-要素饮食补充2%谷氨酰胺。经过适应期（7天）后，所有大鼠接受腹部放疗，每日剂量为300cGy，共5次。放疗4天后，对大鼠进行手术切除小肠和结肠，进行组织学评估。结果——ERt组和GRt组大鼠的小肠组织学数据优于CRt组，表现为保留黏膜细胞数量、增加有丝分裂数量和绒毛长度。同时，ERt组绒毛-隐窝关系正常的大鼠数量多于CRt组或GRt组。大肠组织学数据显示，ERt组和GRt组大鼠的隐窝平均长度大于CRt组。结论——在腹部放疗前、放疗期间和放疗后给予大鼠补充谷氨酰胺的聚合饮食或要素饮食，通过支持黏膜结构和恢复，对辐射损伤具有保护作用。
