Frederiks W M, Vogels I M, Fronik G M
Cell Biochem Funct. 1984 Oct;2(4):217-20. doi: 10.1002/cbf.290020407.
The activity of ornithine carbamyl transferase (OCT) and glutamate pyruvate transaminase (GPT) in serum has been correlated with the extent of necrosis 24 h after different periods of ischaemia in rat liver. The extent of necrosis has been quantified as the volume density of necrosis in the total ischaemic liver lobes using tetranitro BT. The GPT-activity in serum is maximal between 1 and 5 h after different periods of ischaemia, whereas OCT reaches its maximum between 5 and 12 h after ischaemia. The total amount of leaked enzyme-activity as well as the peak value give a linear correlation with the extent of necrosis for OCT and GPT. There is a difference between the character of these two enzymes in that a small leakage of GPT does not indicate liver cell necrosis later on. However, the appearance of OCT in the blood, an enzyme localized in the mitochondrial matrix, has a predictive value for the extent of necrosis, likely to occur later on. GPT, an enzyme from the cytoplasm, can also occur in the blood during the reversible stage of liver cell damage.
大鼠肝脏在经历不同时长的缺血后24小时，血清中鸟氨酸氨基甲酰转移酶（OCT）和谷丙转氨酶（GPT）的活性与坏死程度相关。使用四硝基蓝（tetranitro BT）将坏死程度量化为缺血肝叶总体积中坏死的体积密度。血清中的GPT活性在不同时长缺血后的1至5小时达到最大值，而OCT在缺血后5至12小时达到最大值。OCT和GPT泄漏的酶活性总量以及峰值均与坏死程度呈线性相关。这两种酶的特性存在差异，即GPT少量泄漏并不意味着随后会发生肝细胞坏死。然而，线粒体基质中的酶OCT出现在血液中，对随后可能发生的坏死程度具有预测价值。GPT是一种来自细胞质的酶，在肝细胞损伤的可逆阶段也可能出现在血液中。
