Linnane A W, Lukins H B, Molloy P L, Nagley P, Rytka J, Sriprakash K S, Trembath M K
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2082-5. doi: 10.1073/pnas.73.6.2082.
We have developed a new procedure for the detailed molecular mapping of any allele of the yeast (Saccharomyces cerevisiae) mitochondrial genome. The procedure employs a collection of different genetically characterized petite strains whose genomes have been physically defined by molecular hybridization. The map position of an allele is within the DNA segment common to all defined petites that can be shown by marker rescue to retain the locus. The same collection of petites can be used to locate the positions of mitochondrial rRNA and tRNA cistrons and DNA fragments produced by restriction endonucleases.
我们开发了一种新方法,用于对酵母(酿酒酵母)线粒体基因组的任何等位基因进行详细的分子图谱绘制。该方法利用了一组不同的、具有遗传特征的小菌落菌株,其基因组已通过分子杂交进行了物理定义。等位基因的图谱位置位于所有已定义小菌落共有的DNA片段内,通过标记拯救可以证明该片段保留了该基因座。同一组小菌落可用于定位线粒体rRNA和tRNA顺反子以及限制性内切酶产生的DNA片段的位置。