酿酒酵母ρ负突变体中线粒体遗传标记的修饰重组与传递
Modified recombination and transmission of mitochondrial genetic markers in rho minus mutants of Saccharomyces cerevisiae.
作者信息
Boltin-Fukuhara M, Fukuhara H
出版信息
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4608-12. doi: 10.1073/pnas.73.12.4608.
Abstract
A large number of primary petite (rho-) clones were isolated after ethidium bromide mutagenesis of various grande (rho+) strains of S. cerevisiae that contained the mitochondrial genetic markers, CR, ER, OIR (or OIIR), and PR. From the frequency of coretention of markers in the petites, we have deduced a probable circular order of the markers in the grande mitochondrial genome. From these primary clones several series of pure and stable petite clones were obtained and analyzed genetically. (a) In general, the omega allele is retained or lost together with the region carrying both CR and ER markers. (b) The petites that have retained only the CR marker fall into two classes: some have kept the omega allele of the grande strain they issued from; others exhibit a new omega expression. (c) The proportion of diploid petites in petite X grande crosses is independent of the presence of the omega allele. (d) In most cases, the coordinated transmission of markers observed so far in all grande X grande nonpolar corsses does not exist anymore in petites.
摘要
在用溴化乙锭对含有线粒体遗传标记CR、ER、OIR（或OIIR）和PR的各种酿酒酵母大菌落（rho+）菌株进行诱变后，分离出了大量的初级小菌落（rho-）克隆。从小菌落中标记共保留的频率，我们推断出了大菌落线粒体基因组中标记的可能环状顺序。从这些初级克隆中获得了几个系列的纯合且稳定的小菌落克隆，并进行了遗传分析。（a）一般来说，ω等位基因与携带CR和ER标记的区域一起保留或丢失。（b）仅保留CR标记的小菌落分为两类：一些保留了它们所源自的大菌落菌株的ω等位基因；另一些则表现出新的ω表达。（c）小菌落×大菌落杂交中双倍体小菌落的比例与ω等位基因的存在无关。（d）在大多数情况下，迄今为止在所有大菌落×大菌落非极性杂交中观察到的标记协同传递在小菌落中不再存在。
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本文引用的文献
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Biogenesis of Mitochondria: Analysis of Deletion of Mitochondrial Antibiotic Resistance Markers in Petite Mutants of Saccharomyces cerevisiae.线粒体的生物发生：酿酒酵母小菌落突变体中线粒体抗生素抗性标记缺失的分析
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