Hoyer-Fender S, Burfeind P, Hameister H
III. Department of Zoology (Developmental Biology), University of Göttingen, Germany.
Cytogenet Cell Genet. 1995;70(3-4):200-4. doi: 10.1159/000134033.
The mouse cDNA encoding the major protein of the outer dense fibers in sperm tails was isolated by reverse transcription of testicular RNA and amplification with sequence-specific primers. Sequencing of a genomic clone obtained by inverse PCR yielded the 5' untranslated region. The transcription starting point was verified by primer extension. The putative proteins encoded by Odf1 in mouse and by ODF1 in rat and man are very similar. A total of 15 amino acids in the C-terminal region were deleted in the mouse protein, compared with the rat protein. Through in situ hybridization to metaphase chromosomes, the Odf1 gene was localized to mouse chromosome 15 region B2-C. The chromosomal localization of the Odf1 gene extends the hitherto known linkage group consisting of MYC (Myc), PVT1 (Pvt1), GPT (Gpt), and TG (Tg) common to human chromosome 8 and mouse chromosome 15 in the proximal direction of both chromosomes. The linkage group now extends from band q24 to band q22 of human chromosome 8 and from region D2-E to region B2-C of mouse chromosome 15.
通过睾丸RNA的逆转录和序列特异性引物扩增，分离出编码精子尾部外致密纤维主要蛋白的小鼠cDNA。通过反向PCR获得的基因组克隆测序得到5'非翻译区。通过引物延伸验证转录起始点。小鼠中由Odf1编码以及大鼠和人类中由ODF1编码的推定蛋白非常相似。与大鼠蛋白相比，小鼠蛋白的C末端区域总共缺失了15个氨基酸。通过与中期染色体的原位杂交，将Odf1基因定位到小鼠染色体15的B2-C区域。Odf1基因的染色体定位在两条染色体的近端方向上扩展了迄今已知的由人类染色体8和小鼠染色体15共有的由MYC（Myc）、PVT1（Pvt1）、GPT（Gpt）和TG（Tg）组成的连锁群。该连锁群现在从人类染色体8的q24带延伸到q22带，从小鼠染色体15的D2-E区域延伸到B2-C区域。
