前列腺素对大鼠颌下唾液腺酪氨酰蛋白硫酸转移酶活性的影响。

Effect of prostaglandins on tyrosylprotein sulfotransferase activity in rat submandibular salivary glands.

Kasinathan C, Sundaram R, William S

Dental Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, University Heights, Newark 07103-2400, USA.

Gen Pharmacol. 1995 May;26(3):577-80. doi: 10.1016/0306-3623(94)00224-b.

Abstract

Tyrosylprotein sulfotransferase (TPST) is a key enzyme in the processing of several secretory proteins, including those found in saliva. In this report, the effect of prostaglandins (PG) on TPST activity in submandibular salivary gland was investigated. 2. The results revealed that PGE2 exhibited TPST stimulatory activity with a 1.5-fold stimulation at 100 microM concentration and a half maximal stimulation at 50 microM. The PGE2 stimulation was accompanied by an increase in the affinity of TPST towards sulfate acceptor (Km 1.4 microM-->0.12 microM) with little change in Vmax. 3. The TPST activity was also stimulated by two other major prostaglandins of salivary glands, PGF2 alpha and 6-Keto-PGF 1 alpha, however to lesser extent, 22 and 23%, respectively. Arachidonic acid, an intermediate prostaglandin precursor, had no effect on TPST activity. 4. The results suggest that prostaglandins and in particular PGE2 may play a role in the regulation of TPST catalyzed secretory protein tyrosine sulfation in salivary glands.

摘要

酪氨酰蛋白磺基转移酶（TPST）是几种分泌蛋白加工过程中的关键酶，包括唾液中的分泌蛋白。在本报告中，研究了前列腺素（PG）对下颌下唾液腺中TPST活性的影响。2. 结果显示，PGE2表现出TPST刺激活性，在100微摩尔浓度下刺激1.5倍，在50微摩尔浓度下达到最大刺激的一半。PGE2刺激伴随着TPST对硫酸根受体亲和力的增加（Km从1.4微摩尔变为0.12微摩尔），而Vmax变化不大。3. 唾液腺的另外两种主要前列腺素PGF2α和6-酮-PGF1α也刺激TPST活性，但程度较小，分别为22%和23%。花生四烯酸，一种中间前列腺素前体，对TPST活性没有影响。4. 结果表明，前列腺素尤其是PGE2可能在唾液腺中TPST催化的分泌蛋白酪氨酸硫酸化调节中发挥作用。