影响幽门螺杆菌在液体培养基中生长及药敏试验的因素。
Factors affecting growth and susceptibility testing of Helicobacter pylori in liquid media.
作者信息
Coudron P E, Stratton C W
机构信息
Pathology and Laboratory Medicine Service/113, McGuire Veterans Affairs Medical Center, Richmond, Virginia 23249-0001, USA.
出版信息
J Clin Microbiol. 1995 Apr;33(4):1028-30. doi: 10.1128/jcm.33.4.1028-1030.1995.
Abstract
In order to increase the database for in vitro growth and/or susceptibility testing in liquid media, we evaluated the growth of Helicobacter pylori in broth media containing 5% sheep blood. We also compared the effect of bismuth on the growth of H. pylori in broth media containing 10% fetal calf serum with the effect on growth in media containing 0.5% starch. In contrast to the result seen with agar, we found that sheep blood, whether whole or laked, inhibited the growth of H. pylori in broth media. In addition, we found that bismuth inhibited growth in media with starch but that this inhibition was negated in media with serum.
摘要
为了增加用于液体培养基中体外生长和/或药敏试验的数据库，我们评估了幽门螺杆菌在含5%羊血的肉汤培养基中的生长情况。我们还比较了铋对含10%胎牛血清的肉汤培养基中幽门螺杆菌生长的影响与对含0.5%淀粉的培养基中生长的影响。与在琼脂上观察到的结果相反，我们发现羊血，无论是全血还是溶血，都会抑制幽门螺杆菌在肉汤培养基中的生长。此外，我们发现铋会抑制含淀粉培养基中的生长，但在含血清的培养基中这种抑制作用会被抵消。
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