通过质粒含量、血清分型和脉冲场凝胶电泳对需要脯氨酸、瓜氨酸和尿嘧啶的淋病奈瑟菌分离株进行鉴别。
Differentiation of Neisseria gonorrhoeae isolates requiring proline, citrulline, and uracil by plasmid content, serotyping, and pulsed-field gel electrophoresis.
作者信息
Ng L K, Carballo M, Dillon J A
机构信息
National Laboratory for Sexually Transmitted Diseases, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario.
出版信息
J Clin Microbiol. 1995 Apr;33(4):1039-41. doi: 10.1128/jcm.33.4.1039-1041.1995.
Abstract
A combination of DNA macrorestriction analysis using pulsed-field gel electrophoresis and a serotyping method using three panels of monoclonal antibody was used to discriminate 43 epidemiologically unrelated Neisseria gonorrhoeae isolates requiring proline, citrulline, and uracil (PCU-) into 35 groups. This indicates that PCU- isolates of N. gonorrhoeae are not clonal.
摘要
采用脉冲场凝胶电泳进行DNA宏观限制性分析，并结合使用三组单克隆抗体的血清分型方法，对43株流行病学上不相关的需要脯氨酸、瓜氨酸和尿嘧啶（PCU-）的淋病奈瑟菌分离株进行区分，将其分为35组。这表明淋病奈瑟菌的PCU-分离株不是克隆性的。
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2
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J Clin Microbiol. 1993 Oct;31(10):2831-3. doi: 10.1128/jcm.31.10.2831-2833.1993.
3
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5
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