Schnyder-Candrian S, Strieter R M, Kunkel S L, Walz A
Theodor Kocher Institute, University of Bern, Switzerland.
J Leukoc Biol. 1995 Jun;57(6):929-35. doi: 10.1002/jlb.57.6.929.
The two chemotactic cytokines interleukin-8 (IL-8) and epithelial neutrophil activating protein 78 (ENA-78) were recently shown to be potent chemoattractants and activators of neutrophil function and to be present in certain inflammatory diseases. We have studied the effects of recombinant and natural interferon-alpha (IFN-alpha) and of recombinant interferon gamma (rIFN-gamma) on the production of IL-8 and ENA-78 in lipopolysaccharide- and interleukin-1-stimulated human monocytes. Both types of interferons showed a strong, concentration-dependent inhibition of neutrophil-stimulating bioactivity. Similarly, the secretion of IL-8 and ENA-78 was also inhibited by up to 73%. Northern blot experiments demonstrated that IFN-alpha decreases the steady-state levels of IL-8 and ENA-78 mRNA in monocytes, suggesting that IFN-alpha as well as IFN-gamma may control the expression of neutrophil chemotactic cytokines at the mRNA level.
最近研究发现，两种趋化性细胞因子白细胞介素-8（IL-8）和上皮中性粒细胞激活蛋白78（ENA-78）是中性粒细胞功能的有效趋化因子和激活剂，且存在于某些炎症性疾病中。我们研究了重组和天然α干扰素（IFN-α）以及重组γ干扰素（rIFN-γ）对脂多糖和白细胞介素-1刺激的人单核细胞中IL-8和ENA-78产生的影响。两种类型的干扰素均表现出对中性粒细胞刺激生物活性的强烈、浓度依赖性抑制。同样，IL-8和ENA-78的分泌也被抑制了高达73%。Northern印迹实验表明，IFN-α降低了单核细胞中IL-8和ENA-78 mRNA的稳态水平，这表明IFN-α以及IFN-γ可能在mRNA水平上控制中性粒细胞趋化性细胞因子的表达。
